Experiment Overview
| Repository ID: | FR-FCM-Z5CM | Experiment name: | Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer | MIFlowCyt score: | 64.91% |
| Primary researcher: | James Kim | PI/manager: | James Kim | Uploaded by: | James Kim |
| Experiment dates: | 2021-11-01 - 2021-12-13 | Dataset uploaded: | May 2022 | Last updated: | Mar 2024 |
| Keywords: | [Extracellular vesicles] [Cytoflex] [single-molecule cytometry] [Cellstream] [Limit of detection] | Manuscripts: | |||
| Organizations: |
University of Washington, Chemistry, Seattle, (USA)
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| Purpose: | Compare the characterization of DiFi EVs using CytoFlex and Cellstream for EV characterization?and using the single molecule flow cytometer to determine the limits of detection and sizing for both systems, and compare measurements of EV concentration, size, and protein copy numbers of the DiFi cell marker EGFR and the EV tetraspanins CD9, CD63, and CD81 between the three systems. | ||||
| Conclusion: | Based on the LoD of each flow cytometer, the detected population varies. | ||||
| Comments: | None | ||||
| Funding: | NIH grants and VA Merit grants | ||||
| Quality control: | MeCP2-GFP/Ai14 cortical FACS | We followed the ISEV guideline for the controls. | |||
